Journal: Tropical Animal Health and Production

Article Title: Morphological characterization and immunohistochemical detection of the proinflammatory cytokines IL-1β, IL-17A, and TNF-α in lung lesions associated with contagious bovine pleuropneumonia

doi: 10.1007/s11250-016-0994-9

Figure Lengend Snippet: Semi-quantitative assessment of inflammatory cytokines in the lung of infected and uninfected animals

Article Snippet: Bovine IL-1β, IL-17A, and TNF-α were detected by using biotinylated rabbit anti-bovine IL-1β polyclonal antibody (article no. ab23778, Abcam, UK), biotinylated rabbit anti-bovine IL-17A polyclonal antibody (article no. PBB0277B-050, Kingfisher Biotech, Inc., USA), and biotinylated rabbit anti-bovine TNF-α polyclonal antibodies (article no. AHP852B, AbD Serotec, Germany), respectively.

Techniques: Infection, Negative Control

Journal: Tropical Animal Health and Production

Article Title: Morphological characterization and immunohistochemical detection of the proinflammatory cytokines IL-1β, IL-17A, and TNF-α in lung lesions associated with contagious bovine pleuropneumonia

doi: 10.1007/s11250-016-0994-9

Figure Lengend Snippet: Representative qualitative results of the detection of the cytokines IL-1β, IL-17A, and TNF-α. The following polyclonal antibodies were used. a Anti- IL-1β: Lung: control animal; b Anti- IL-1β: Lung: acute CBPP lesion; c Anti- IL-17A: Lung: control animal; d Anti- IL-17A: Lung: acute CBPP lesion; e Anti- TNF-α: Lung: control animal; f Anti- TNF-α: Lung: acute CBPP lesion; g Anti- Mycoplasma : Lung: control animal; h Anti- Mycoplasma : Lung: acute CBPP lesion. Black bars in the lower right corner of images in a – f is the size standard representing 100 μm, black bars in images of g , h represent 20 μm. Arrows indicate positive immunoreactive signals ( reddish-brown )

Article Snippet: Bovine IL-1β, IL-17A, and TNF-α were detected by using biotinylated rabbit anti-bovine IL-1β polyclonal antibody (article no. ab23778, Abcam, UK), biotinylated rabbit anti-bovine IL-17A polyclonal antibody (article no. PBB0277B-050, Kingfisher Biotech, Inc., USA), and biotinylated rabbit anti-bovine TNF-α polyclonal antibodies (article no. AHP852B, AbD Serotec, Germany), respectively.

Techniques: Control

Journal: Cells

Article Title: Ets Family Transcription Factor Fli-1 Promotes Leukocyte Recruitment and Production of IL-17A in the MRL/Lpr Mouse Model of Lupus Nephritis

doi: 10.3390/cells9030714

Figure Lengend Snippet: Serum cytokine levels and renal mRNA expression related to interleukin (IL)-6 and IL-17A cytokine signaling. ( A ) Serum IL-6 and IL-17A concentrations (pg/mL) in adult (4 to 10 months old) wild type (WT) ( n = 11–15) and MRL/lpr Fli-1 +/− mice ( n = 15 per group). ( B ) Relative expression of cytokines and related signaling molecules in the kidney of adult (4 to 9 months old) MRL/lpr Fli-1 +/− ( n = 3–11) vs. WT mice ( n = 4–13); * p < 0.05 is considered as significant.

Article Snippet: IL-17A was detected in tissues with rabbit polyclonal anti-IL-17A antibodies (Abcam) and the standard ultra-sensitive avidin-biotin complex (ABC) method (Vector).

Techniques: Expressing

Journal: Cells

Article Title: Ets Family Transcription Factor Fli-1 Promotes Leukocyte Recruitment and Production of IL-17A in the MRL/Lpr Mouse Model of Lupus Nephritis

doi: 10.3390/cells9030714

Figure Lengend Snippet: IL-17 staining of MRL/lpr Fli-1 +/+ (WT) and MRL/lpr Fli-1 +/- mice. ( A , B ) Immunostaining of IL-17A in the kidney tissue from WT MRL/lpr and MRL/lpr Fli-1 +/− mice (4 months old or older) and pathology scores in ( C ) ( n = 7–9 per groups) are shown; * p < 0.05 is considered as significant.

Article Snippet: IL-17A was detected in tissues with rabbit polyclonal anti-IL-17A antibodies (Abcam) and the standard ultra-sensitive avidin-biotin complex (ABC) method (Vector).

Techniques: Staining, Immunostaining

Journal: Cells

Article Title: Ets Family Transcription Factor Fli-1 Promotes Leukocyte Recruitment and Production of IL-17A in the MRL/Lpr Mouse Model of Lupus Nephritis

doi: 10.3390/cells9030714

Figure Lengend Snippet: Detection of CD3 + IL-17A + and CD4 + IL-17A + immune cells in the kidney of MRL/lpr Fli-1 +/+ (WT) and MRL/lpr Fli-1 +/− mice using FITC-conjugated anti-CD3, anti-CD4, and Alexa Fluor 647-labeled IL-17 antibodies. Detection of CD3 + IL-17A + and CD4 + IL-17A + cells in renal tissue from WT MRL/lpr and MRL/lpr Fli-1 +/− mice (4 months old or older) is shown ( A – D ). Cell counts of CD3 + IL-17 + and CD4 + IL-17 + leukocytes and total counts from ten random high-power fields (HPFs) ( n = 6–10 per groups) are shown ( E ); * p < 0.05 is considered as significant.

Article Snippet: IL-17A was detected in tissues with rabbit polyclonal anti-IL-17A antibodies (Abcam) and the standard ultra-sensitive avidin-biotin complex (ABC) method (Vector).

Techniques: Labeling